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In vitro & in vivo Pharmacology Services

To gain profound and relevant preclinical data on efficacy and potency, in-depth knowledge of the corresponding disease is a key success factor. GBA Pharma has a proven track record on conducting in vitro and in vivo studies on potency, selectivity and efficacy in several indication areas.

In vitro pharmocology assays

5α Reductase Inhibitors
  • Human recombinant isoenzymes type I und II
Aromatase Inhibitors
  • Recombinant human aromatase (CYP 19)
CYP17 Inhibitors
  • Human recombinant CYP 17
LHRH-A(nta)gonists
  • Interaction with LHRH-induced LH secretion in primary rat pituitary cells
Gluco- and Mineralo­corticoid Biosynthesis Inhibitors
  • Human steroid-secreting adrenocortical cell line
Inflammation
  • Cytokine induction (PMBC, monocytes), Luminex
  • Histamine release (mastocytes)
Assay Development and Implementation
  • Literature search
  • Validation with reference compounds
  • Implementation

In vivo pharmocology models

Our in vivo team is experienced in designing and setting up studies for the below listed disease models. We offer customized studies to address specific project requirement and need at least 2 month prior notice before starting any of the below studies.

Model Development and Implementation
  • Literature search
  • Application to animal welfare authorities
  • Validation with reference compounds
  • Implementation
Diabetes / Metabolic Syndrome Models
  • Induced Models:
    –   STZ Mice/Rats
    –   DIO Mice/Rat
  • Genetic Models:
    –   ob/ob Mice
    –   db/db Mice
    –   Zucker/ZDF Rat
Fibrosis Models
  • Liver Fibrosis:
    –   Bile duct ligation in rats
    –   Thioacetamide-induced liver fibrosis in rats
  • Kidney Fibrosis:
    –   Unilateral ureter ligation in mice or rats
Cancer
  • In vivo model (immunodeficient mice)
  • Principle:
    s.c. tumor xenograft model
  • General Readouts:
    Tumor incidence, tumor size, tumor growth inhibition, terminal tumor weight, Occurence of metastases (postmortal)
Non-alcoholic Fatty Liver Disease
  • In vivo model (rat or mouse)
  • Principle:
    NAFLD induced by specific diets (high fat, high cholesterol)
  • General Readouts:
    Liver weight, clinical chemistry (e.g. ALT, AST, T-BIL, LDL, cholesterol etc.) in plasma, metabolic profile in plasma and tissue
  • Specific Readouts:
    Diabetic status, insulin resistance, triglycerides, LDL, inflammatory cytokines
Steroid Endocrinology
  • 5a Reductase, Androgen Receptor:
    Readout: (anti-) androgenic activity as indicated by effects on prostate growth in castrated juvenile or adult rats
  • Aromatase, Estrogen Receptor:
    Readout: (anti-) estrogenic activity as indicated by effects on uterine growth in juvenile rats or ovariectomised adult rats
Steroid Endocrinology
  • LHRH-Receptor:
    Readout: LH levels in castrated rats
  • Aldosterone Synthase:
    Readout: aldosterone levels in ACTH-stimulated rats
  • CYP17 (17a-hydroxylase/ 17,20 lyase/ 17,20 desmolase):
    Readout: testosterone levels in adult rats

Leveraging our experience allows us to design, set-up and perform in vitro and in vivo pharmacology studies according to the specific requirements of your project.

Brands of
GBA GROUP PHARMA